This subsection of the 'Structure' section is used to indicate the positions of experimentally determined helical regions within the protein sequence.
using the generator polynomial: x64 + x4 + x3 + x + 1.
The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:
This effect of θ was demonstrated both indirectly, through the effect of θ on bacterial mutation frequencies, and directly, using the yeast three-hybrid assay.The beneficial effect of θ was noted in particular for While large effects of θ were observed in the case of mutant DnaQ proteins, smaller, but significant effects were also observed with the It is interesting that the replication complex of gram-negative bacteria is characterized by both a θ subunit and a separate proofreading subunit (i.e., not part of the polymerase polypeptide).This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.
The DNA polymerase III (Pol III) holoenzyme (HE) is the major chromosomal replication enzyme in In contrast, the role of the θ subunit of the Pol III core is unknown. from the sequence.This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).
Manual assertion inferred from combination of experimental and computational evidence These are stable identifiers and should be used to cite UniProtKB entries. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).
This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.
This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.
This indicates the type of evidence that supports the existence of the protein. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].The Biological General Repository for Interaction Datasets (BioGRID)ComplexPortal: manually curated resource of macromolecular complexesSWISS-MODEL Repository - a database of annotated 3D protein structure modelsRelative evolutionary importance of amino acids within a protein sequenceevolutionary genealogy of genes: Non-supervised Orthologous GroupsThe HOGENOM Database of Homologous Genes from Fully Sequenced OrganismsGene3D Structural and Functional Annotation of Protein FamiliesIntegrated resource of protein families, domains and functional sitesDNA Data Bank of Japan; a nucleotide sequence databaseProtein sequence database of the Protein Information ResourceEnsembl bacterial and archaeal genome annotation projectEchoBASE - an integrated post-genomic database for E. coliProtoNet; Automatic hierarchical classification of proteinsMobiDB: a database of protein disorder and mobility annotationsYou are using a version of browser that may not display all the features of this website. The version number for both the entry and the canonical sequence are also displayed.
Being the primary holoenzyme involved in replication activity, the DNA Pol III holoenzyme also has proofreading capabilities that corrects replication mistakes by means of exonu 1992 May 1; 69 (3):425–437. The polymerase is tethered to the template via the sliding clamp processivity factor. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.An evidence describes the source of an annotation, e.g. Lancy ED, Lifsics MR, Kehres DG, Maurer R. Isolation and characterization of mutants with deletions in dnaQ, the gene for the editing subunit of DNA polymerase III in Salmonella typhimurium. The exact function of the theta subunit is unknown. The DNA polymerase III (Pol III) holoenzyme (HE) is the major chromosomal replication enzyme in Escherichia coli (19, 22, 30, 31).The enzyme is composed of 17 subunits, 10 of which are distinct (19, 31).HE contains two polymerase core molecules, each consisting of an α, ε, and θ subunit arranged in the linear order α-ε-θ. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau.
This subsection of the 'Interaction' section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the 'Function' section).